A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences
作者： Ai-Sheng Xiong , Quan-Hong Yao , Ri-He Peng , Xian Li , Hui-Qin Fan
DOI: 10.1093/NAR/GNH094
关键词: Multiple displacement amplification 、 Polymerase cycling assembly 、 Base pair 、 Oligonucleotide 、 Molecular biology 、 Computational biology 、 Biology 、 Primer (molecular biology) 、 Polymerase chain reaction 、 Primer dimer 、 DNA polymerase
摘要: … High-fidelity Taq DNA polymerase Pfu was purchased from Promega (Madison, WI). DNA marker (λ-DNA Hind III DNA marker) was obtained from New England Biolabs (Beverly, MA). …
oup.com
paperity.org
core.ac.uk
oxfordjournals.org参考文章(27)
Dübel S, Welschof M, Breitling F, Braunagel M, Little M, Hayashi N, Zewe M, Simultaneous mutagenesis of antibody CDR regions by overlap extension and PCR. BioTechniques. ,vol. 17, pp. 310- 315 ,(1994)
P. J. Dillon, C. A. Rosen, A rapid method for the construction of synthetic genes using the polymerase chain reaction. BioTechniques. ,vol. 9, pp. 298- 300 ,(1990)
E Jay, D MacKnight, C Lutze-Wallace, D Harrison, P Wishart, W Y Liu, V Asundi, L Pomeroy-Cloney, J Rommens, L Eglington, Chemical synthesis of a biologically active gene for human immune interferon-gamma. Prospect for site-specific mutagenesis and structure-function studies. Journal of Biological Chemistry. ,vol. 259, pp. 6311- 6317 ,(1984) , 10.1016/S0021-9258(20)82142-5
M.D. Edge, A.R. Greene, G.R. Heathcliffe, V. E. Moore, N.J. Faulkner, R. Camble, N.N. Petter, P. Trueman, W. Schuch, J. Hennam, T.C. Atkinson, C.R. Newton, A.F. Markham, Chemical synthesis of a human interferon-α2, gene and its expression in Escherichia coli Nucleic Acids Research. ,vol. 11, pp. 6419- 6435 ,(1983) , 10.1093/NAR/11.18.6419
Chrisostomos Prodromou, Laurence H. Pearl, Recursive PCR: a novel technique for total gene synthesis. Protein Engineering. ,vol. 5, pp. 827- 829 ,(1992) , 10.1093/PROTEIN/5.8.827
Richard B. Ciccarelli, Paul Gunyuzlu, James Huang, Charles Scott, Fred T. Oakes, Construction of synthetic genes using PCR after automated DNA synthesis of their entire top and bottom strands Nucleic Acids Research. ,vol. 19, pp. 6007- 6013 ,(1991) , 10.1093/NAR/19.21.6007
John Smith, E. Cook, Ian Fotheringham, Sally Pheby, Roger Derbyshire, Michael A.W. Eaton, Mike Doel, David M.J. Lilley, John F. Pardon, Thakor Patel, Hilary Lewis, Leslie D. Bell, Chemical synthesis and cloning of a gene for human β-urogastrone Nucleic Acids Research. ,vol. 10, pp. 4467- 4482 ,(1982) , 10.1093/NAR/10.15.4467
H. O. Smith, C. A. Hutchison, C. Pfannkoch, J. C. Venter, Generating a synthetic genome by whole genome assembly: φX174 bacteriophage from synthetic oligonucleotides Proceedings of the National Academy of Sciences of the United States of America. ,vol. 100, pp. 15440- 15445 ,(2003) , 10.1073/PNAS.2237126100
Paul M. Sharp, Therese M.F. Tuohy, Krzysztof R. Mosurski, Codon usage in yeast: cluster analysis clearly differentiates highly and lowly expressed genes Nucleic Acids Research. ,vol. 14, pp. 5125- 5143 ,(1986) , 10.1093/NAR/14.13.5125
Brian S. Sproat, Michael J. Gait, Chemical synthesis of a gene for somatomedin C Nucleic Acids Research. ,vol. 13, pp. 2959- 2977 ,(1985) , 10.1093/NAR/13.8.2959