Generating a synthetic genome by whole genome assembly: φX174 bacteriophage from synthetic oligonucleotides
作者： H. O. Smith , C. A. Hutchison , C. Pfannkoch , J. C. Venter
关键词:
摘要: We have improved upon the methodology and dramatically shortened the time required for accurate assembly of 5- to 6-kb segments of DNA from synthetic oligonucleotides. As a test of …
harvard.edu
fbae.org 参考文章(11)
M. K. Cho, GENETICS:Ethical Considerations in Synthesizing a Minimal Genome Science. ,vol. 286, pp. 2087- 2090 ,(1999) , 10.1126/SCIENCE.286.5447.2087
T Sekiya, T Takeya, E L Brown, R Belagaje, R Contreras, H J Fritz, M J Gait, R G Lees, M J Ryan, H G Khorana, K E Norris, Total synthesis of a tyrosine suppressor transfer RNA gene. XVI. Enzymatic joinings to form the total 207-base pair-long DNA. Journal of Biological Chemistry. ,vol. 254, pp. 5787- 5800 ,(1979) , 10.1016/S0021-9258(18)50482-8
L.J. McBride, M.H. Caruthers, An investigation of several deoxynucleoside phosphoramidites useful for synthesizing deoxyoligonucleotides Tetrahedron Letters. ,vol. 24, pp. 245- 248 ,(1983) , 10.1016/S0040-4039(00)81376-3
F. Sanger, A.R. Coulson, T. Friedmann, G.M. Air, B.G. Barrell, N.L. Brown, J.C. Fiddes, C.A. Hutchison, P.M. Slocombe, M. Smith, The nucleotide sequence of bacteriophage φX174 Journal of Molecular Biology. ,vol. 125, pp. 225- 246 ,(1978) , 10.1016/0022-2836(78)90346-7
M. Goulian, A. Kornberg, R. L. Sinsheimer, Enzymatic synthesis of DNA, XXIV. Synthesis of infectious phage phi-X174 DNA. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 58, pp. 2321- 2328 ,(1967) , 10.1073/PNAS.58.6.2321
Jeronimo Cello, Aniko V Paul, Eckard Wimmer, Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence of Natural Template Science. ,vol. 297, pp. 1016- 1018 ,(2002) , 10.1126/SCIENCE.1072266
Arthur Kornberg, Ten Commandments: Lessons from the Enzymology of DNA Replication Journal of Bacteriology. ,vol. 182, pp. 3613- 3618 ,(2000) , 10.1128/JB.182.13.3613-3618.2000
J. P. Huelsenbeck, H. A. Wichman, David M Hillis, M. R. Badgett, James J Bull, Ian J Molineux, A. Gulati, C. Ho, Exceptional Convergent Evolution in a Virus Genetics. ,vol. 147, pp. 1497- 1507 ,(1997) , 10.1093/GENETICS/147.4.1497
Willem P.C. Stemmer, Andreas Crameri, Kim D. Ha, Thomas M. Brennan, Herbert L. Heyneker, Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides. Gene. ,vol. 164, pp. 49- 53 ,(1995) , 10.1016/0378-1119(95)00511-4
Clyde A Hutchison III, Scott N Peterson, Steven R Gill, Robin T Cline, Owen White, Claire M Fraser, Hamilton O Smith, J Craig Venter, Global Transposon Mutagenesis and a Minimal Mycoplasma Genome Science. ,vol. 286, pp. 2165- 2169 ,(1999) , 10.1126/SCIENCE.286.5447.2165