Carbon partitioning in lipids synthesized by Chlamydomonas reinhardtii when cultured under three unique inorganic carbon regimes

摘要: Abstract Inorganic carbon is a fundamental component for microalgal lipid biosynthesis. Understanding how the concentration and speciation of dissolved inorganic (DIC) influences metabolism in microalgae may help researchers optimize production these high value metabolites. Using relatively straight forward methods quantifying free fatty acids (FFAs), mono- (MAG), di- (DAG), tri-acylglycerides (TAG), total cellular (FAME), profiles over time were established Chlamydomonas reinhardtii when grown under three unique regimes. Specifically, cultures sparged with atmospheric air compared to which 5% CO2 (v/v) supplemented 50 mM NaHCO3 just prior medium nitrogen depletion. All conditions exhibited similar depletion medium, FFA MAG being predominant However, precursors quickly reallocated into DAG subsequently TAG after C16 did not accumulate significantly any treatments, whereas C18 content increased throughout both exponential growth stationary phase. began depletion, contributing most overall content. shift towards saturated Results provide insight partitioning compounds organism's changes due N-deplete culturing source availability. The methodologies findings presented here be adapted other organisms industrial relevance.