NF-κB-like factors in the murine brain. Developmentally-regulated and tissue-specific expression

摘要: Abstract NF-κB and related factors are important transducers of external signals to the cell nucleus. They abundant in brain, where they may be significant for regulation gene transcription plasticity-related processes instance, via activation protein kinase C. The subunit composition levels these mouse rat brain other tissues, using an assay based on gel retardation oligonucleotides corresponding κB DNA-element, reported here. Three major κB-binding were observed. Factors I II activated by dissociating agent deoxycholate. DNA cross-linking antibody neutralization experiments suggest that factor is a heterodimer c-Rel p65; p50 p65 (authentic NF-κB), c-Rel; III homodimer (KBF1). All three generally expressed 17-day-old embryo 5-day-old pup, whereas adult rat, expression was more limited showed certain tissue specificity. Factor most only observed brain. detected testis spleen and, small amounts, liver lung. Two minor κB-specific (A B), distinctive spleen, respectively, very slow mobility. Their estimated molecular weights about 125 kDa 95 kDa, respectively. Expression A stable during development. identical previously described brain-specific factor, BETA (Korner et al., Neuron, 3 (1989) 563–572). Thus, pattern activities apparently developmentally regulated tissue-specific particularly adult. In (probably p50/c-Rel heterodimer) BETA) could