A gene encoding rat cholecystokinin. Isolation, nucleotide sequence, and promoter activity.
作者： R J Deschenes , R S Haun , C L Funckes , J E Dixon
DOI: 10.1016/S0021-9258(20)71240-8
关键词: Genetics 、 Chloramphenicol acetyltransferase 、 Polyadenylation 、 Molecular biology 、 Gene 、 Intron 、 Exon 、 Nucleic acid sequence 、 Transcription (biology) 、 Sequence analysis 、 Biology
摘要: Abstract The gene for rat cholecystokinin (CCK) was isolated from a genomic DNA library. transcription unit spans 7 kilobases and is interrupted by two introns. initiator methionine codon lies 2 bases into exon 2; therefore, 1 noncoding exon. initiation site determined using avian myeloblastosis reverse transcriptase, cDNA primer, mRNA medullary thyroid carcinoma. A "TATA"-like sequence precedes the at position -34. polyadenylation mapped nuclease protection assay cRNA generated of 3 region CCK with SP6 bacteriophage RNA polymerase. AT-TAAA found 22 5' to be addition site. Two regions simple repetitive occur within lambda clone, one intron other 4 3' gene. Sequence analysis element distal revealed copies 5'-(AC)n-3', where n 25. 114-base pair predominantly repeating purine-pyrimidine nucleotides separates these d(AC) repeats. Transcriptional control elements were investigated fusing structural encoding chloramphenicol acetyltransferase. Promoter activity transfecting COS-7 cells plasmids containing fusions, followed determining acetyltransferase in cellular extracts. necessary expression fusions 144 transcription.
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