Fiber density of electrospun gelatin scaffolds regulates morphogenesis of dermal-epidermal skin substitutes.

摘要: Porous, nowoven fibrous gelatin scaffolds were prepared using electrospinning. Electrospun with varying fiber diameter, interfiber distance, and porosity fabricated by altering the concentration of electrospinning solution. Solution was a significant predictor higher solution correlated larger diameters distances. The potential electrospun as scaffolding material for dermal epidermal tissue regeneration also evaluated. Interfiber distances >5.5 μm allowed deeper penetration human fibroblasts into scaffold, whereas cells in more densely packed fibers able to infiltrate only upper regions. Scaffolds ≤10 exhibited well-stratified layers including continuous basal keratinocyte layer. These shown form keratinized layer like normal skin, which acts barrier infection fluid loss. between 5 10 appear yield most favorable skin substitute vitro, demonstrating high cell viability, optimal organization, excellent formation. results demonstrate feasibility scaffold dermal–epidermal composite substitutes. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008