The In Vitro Replication of Adenovirus DNA

摘要: Adenovirus (Ad) DNA is a linear double-stranded molecule of approximately 36000 bp with covalently bound 55-kilodalton (K) terminal protein (TP) linked to the 5′ terminus each strand (Robinson et al. 1973; Rekosh 1977; Carusi 1977). Ad has inverted repeats about 100 exact length depending on serotype (Steenbergh Arrand and Roberts 1979; Shinagawa Padmanabhan 1979). A highly conserved sequence extending from nucleotides 9 22 either end may serve as recognition site for proteins involved in replication (Shinagawa 1980; Tolun In addition, 5′-terminal dCMP residue all serotypes sequenced. The TP attached via phosphodiester bond between β-hydroxyl group serine 5′-phosphate (Desiderio Kelly 1981). synthesized an 80K precursor (pTP) which processed late infection 55K form by Ad-coded protease (Challberg 1981; Stillman Lichy 1982 b). Mapping vitro translation mRNAs hybridized restriction fragments (Stillman 1981) showed it be viral gene product encoded map coordinates 11 30 E2B region. Subsequent determination this region (Alestrom 1982), together partial amino acid studies (Smart 1982) further localized open reading frame 28.4 23.2 leftward strand.