Selective inhibition of mutant Ha-ras mRNA expression by antisense oligonucleotides.
作者： B.P. Monia , J.F. Johnston , D.J. Ecker , M.A. Zounes , W.F. Lima
DOI: 10.1016/S0021-9258(19)88650-7
关键词: DNA 、 Reporter gene 、 Oligonucleotide 、 Nucleic acid thermodynamics 、 Molecular biology 、 Wild type 、 Mutant 、 Mutation 、 Point mutation 、 Biology
摘要: A biological reporter gene assay was employed to determine the crucial parameters for maximizing selective targeting of a Ha-ras codon 12 point mutation (G----T) using phosphorothioate antisense oligonucleotides. We have tested series oligonucleotides ranging in length between 5 and 25 bases, each centered around mutation. Our results indicate that this can be achieved with oligonucleotides, but selectivity is critically dependent upon oligonucleotide concentration. The maximum observed experiments, 5-fold 17-base oligonucleotide, closely predicted by simple thermodynamic model relates fraction mutant wild type target bound as function concentration affinity. These suggest analysis oligonucleotide/target interactions useful predicting specificity an targeted single base
sci-hub.st 参考文章(62)
Habener Jf, Brasier Ar, Tate Je, Optimized use of the firefly luciferase assay as a reporter gene in mammalian cell lines. BioTechniques. ,vol. 7, pp. 1116- 1122 ,(1989)
Joseph D. Puglisi, Ignacio Tinoco, Absorbance melting curves of RNA. Methods in Enzymology. ,vol. 180, pp. 304- 325 ,(1989) , 10.1016/0076-6879(89)80108-9
M. Kozak, Structural features in eukaryotic mRNAs that modulate the initiation of translation. Journal of Biological Chemistry. ,vol. 266, pp. 19867- 19870 ,(1991) , 10.1016/S0021-9258(18)54860-2
Gerald Zon, Oligonucleotide Analogues as Potential Chemotherapeutic Agents Pharmaceutical Research. ,vol. 5, pp. 539- 549 ,(1988) , 10.1023/A:1015985728434
R S Morrison, Suppression of basic fibroblast growth factor expression by antisense oligodeoxynucleotides inhibits the growth of transformed human astrocytes. Journal of Biological Chemistry. ,vol. 266, pp. 728- 734 ,(1991) , 10.1016/S0021-9258(17)35232-8
M.Y. Chiang, H. Chan, M.A. Zounes, S.M. Freier, W.F. Lima, C.F. Bennett, Antisense oligonucleotides inhibit intercellular adhesion molecule 1 expression by two distinct mechanisms. Journal of Biological Chemistry. ,vol. 266, pp. 18162- 18171 ,(1991) , 10.1016/S0021-9258(18)55250-9
C. K. Mirabelli, H. Chan, Ming-Yi Chiang, J. E. E. Shoemaker, C. F. Bennett, Cationic lipids enhance cellular uptake and activity of phosphorothioate antisense oligonucleotides. Molecular Pharmacology. ,vol. 41, pp. 1023- 1033 ,(1992)
T. Saison-Behmoaras, B. Tocqué, I. Rey, M. Chassignol, N. T. Thuong, C. Hélène, Short modified antisense oligonucleotides directed against Ha-ras point mutation induce selective cleavage of the mRNA and inhibit T24 cells proliferation. The EMBO Journal. ,vol. 10, pp. 1111- 1118 ,(1991) , 10.1002/J.1460-2075.1991.TB08051.X
G Duff, Jean Manson, T Brown, Modulation of interleukin 1 beta gene expression using antisense phosphorothioate oligonucleotides Lymphokine research. ,vol. 9, pp. 35- 42 ,(1990)
Elizabeth Taparowsky, Kenji Shimizu, Mitchell Goldfarb, Michael Wigler, Structure and activation of the human N-ras gene Cell. ,vol. 34, pp. 581- 586 ,(1983) , 10.1016/0092-8674(83)90390-2