Evaluation of Chitosan-Based Films Containing Gelatin, Chondroitin 4-Sulfate and ZnO for Wound Healing

摘要: In this work, chitosan-based films containing gelatin and chondroitin-4-sulfate (C4S) with without ZnO particles were produced tested in vitro to investigate their potential wound healing properties. Chitosans from shrimp-head processing waste by alkaline deacetylation of chitin obtain chitosans differing molecular weight degree (80 ± 0.5%). The film-forming solutions (chitosan, C4S gelatin) suspension showed no toxicity towards fibroblasts or keratinocytes. Chitosan was able agglutinate red blood cells, induced hemolysis. Film components released into solution when incubated PBS as demonstrated protein sugar determination. These data suggest that a stable, film low an ability release would be establish biocompatible microenvironment for cell growth. Chitosan-based significantly increased the percentage (wound contraction 65 86%) skin full-thickness excision compared control (51%), after 6 days. Moreover, histological analysis granulation tissue chitosan chitosan/gelatin/C4S/ZnO films. biopolymer composites could used improved biomedical applications such dressings, giving them enhanced