The plasma membrane oxidase NtrbohD is responsible for AOS production in elicited tobacco cells
作者： Francoise Simon-Plas , Taline Elmayan , Jean-Pierre Blein
DOI: 10.1046/J.1365-313X.2002.01342.X
关键词: Biology 、 Elicitor 、 Nicotiana tabacum 、 Biochemistry 、 Cell biology 、 Extracellular 、 NADPH oxidase 、 Oxidase test 、 Complementary DNA 、 Gene expression 、 Cell culture
摘要: Summary A cDNA encoding a protein, NtrbohD, located on the plasma membrane and homologue to flavocytochrome of neutrophil NADPH oxidase, was cloned in tobacco. The corresponding mRNA accumulated when tobacco leaves cells were treated with fungal elicitor cryptogein. After elicitation cryptogein, transformed antisense constructs NtrbohD showed same extracellular alkalinization as control, but no longer produced active oxygen species (AOS). This work represents first demonstration function gp91–phox AOS production elicited cells.
sci-hub.se 参考文章(39)
N. T. Keen, Pathogenic Strategies of Fungi Springer, Berlin, Heidelberg. pp. 171- 188 ,(1986) , 10.1007/978-3-642-71652-2_17
P. Ricci, F. Panabieres, P. Bonnet, N. Maia, M. Ponchet, J.-C. Devergne, A. Marais, L. Cardin, M. L. Milat, J. P. Blein, Proteinaceous Elicitors of Plant Defense Responses Springer, Dordrecht. pp. 121- 135 ,(1993) , 10.1007/978-94-011-1737-1_34
P. Ricci, P. Bonnet, P. Abad, P. M. Molot, P. Mas, M. Bruneteau, I. Fabre, O. Lhomme, G. Michel, Differential Elicitation Activities of Fractions from Phytophthora Spp. On Several Host-Plants NATO advanced research workshop on biology and molecular biology of plant-pathogen interactions. ,vol. 1, pp. 191- 196 ,(1986) , 10.1007/978-3-642-82849-2_16
E. Tavernier, D. Wendehenne, J. P. Blein, A. Pugin, Involvement of Free Calcium in Action of Cryptogein, a Proteinaceous Elicitor of Hypersensitive Reaction in Tobacco Cells. Plant Physiology. ,vol. 109, pp. 1025- 1031 ,(1995) , 10.1104/PP.109.3.1025
G.P. Bolwell, P. Wojtaszek, Mechanisms for the generation of reactive oxygen species in plant defence – a broad perspective Physiological and Molecular Plant Pathology. ,vol. 51, pp. 347- 366 ,(1997) , 10.1006/PMPP.1997.0129
Przemysław WOJTASZEK, Oxidative burst: an early plant response to pathogen infection Biochemical Journal. ,vol. 322, pp. 681- 692 ,(1997) , 10.1042/BJ3220681
Radhika DESIKAN, Adele REYNOLDS, T. John HANCOCK, J. Steven NEILL, Harpin and hydrogen peroxide both initiate programmed cell death but have differential effects on defence gene expression in Arabidopsis suspension cultures Biochemical Journal. ,vol. 330, pp. 115- 120 ,(1998) , 10.1042/BJ3300115
Taline Elmayan, Herve Vaucheret, Expression of single copies of a strongly expressed 35S transgene can be silenced post-transcriptionally Plant Journal. ,vol. 9, pp. 787- 797 ,(1996) , 10.1046/J.1365-313X.1996.9060787.X
Robert H. Kretsinger, EF-hands reach out. Nature Structural & Molecular Biology. ,vol. 3, pp. 12- 15 ,(1996) , 10.1038/NSB0196-12
Jeff Dangl, Eric Holub, La Dolce Vita: A Molecular Feast in Plant–Pathogen Interactions Cell. ,vol. 91, pp. 17- 24 ,(1997) , 10.1016/S0092-8674(01)80005-2