The small 11kDa nonstructural protein of human parvovirus B19 plays a key role in inducing apoptosis during B19 virus infection of primary erythroid progenitor cells

摘要: Human parvovirus B19 (B19V) infection shows a strong erythroid tropism and drastically destroys progenitor cells, thus leading to most of the disease outcomes associated with B19V infection. In this study, we systematically examined 3 nonstructural proteins, 7.5kDa, 11kDa, NS1, for their function in inducing apoptosis transfection primary ex vivo–expanded comparison induced during Our results show that 11kDa is more significant inducer than whereas 7.5kDa does not induce apoptosis. Furthermore, determined caspase-10, an initiator caspase death receptor signaling, active apoptotic progenitors by NS1 as well More importantly, cytoplasm-localized expressed at least 100 times nucleus-localized protein level cells infected B19V; inhibition expression using antisense oligos targeting specifically 11kDa-encoding mRNAs reduces significantly cells. Taken together, these demonstrate contributes cell