Characterization of a low molecular weight suppressor of lymphocyte proliferation from guinea pig 12C leukemia cells

作者: Daniel P. Collins , Daniel J. Cook , Manuel J. Ricardo

DOI: 10.1016/0008-8749(87)90087-6

关键词: ThymidineCell activationBiochemistryMolecular biologyLymphocyte proliferationBiologyGuinea pigCell growthPronaseLymphocyteIn vivoImmunology

摘要: Abstract Conditioned medium (CM) from 24-hr culture of guinea pig L2C B lymphoblastic leukemia cells contained an inhibitor(s) mitogen- and antigen-stimulated proliferation syngeneic (strain 2 pigs), allogeneic (Hartley xenogeneic (Balb/c mouse, NZW rabbit) lymphocytes. The several lymphoid nonlymphoid cell lines also was inhibited in the presence CM. CM not toxic to any cultures studied. mitogen-stimulated lymphocytes when added up 52 hr after addition mitogen. Normal responsiveness mitogens could be restored by washing CM-treated with during first 6 culture. exogenous IL-2 lymphocyte did overcome CM-mediated suppression or proliferation. IL-2-dependent murine CTLL-2 cells. Preincubation inhibit capacity these release exposure antiproliferative activity stable heating at low pH (100 °C, 10 min, 4.0), resistant treatment papain, pronase, DNase, RNase bind Con A-Sepharose. Incubation indomethacin inhibitor(s). had apparent molecular weight 500–3500 Da as determined dialysis ultrafiltration analysis. inhibitory recovered organic phase extraction chloroform:methanol eluted distinct thymidine standard gel filtration on Sephadex-G 25. These data suggest that is a nonspecific, weight, lipid-like component (not prostaglandin) exerts its effects subsequent activation. appear suppress other biologic functions associated activation, such secretion. inhibitor may important promoting tumor survival vivo suppressing potential anti-tumor cellular immune responsiveness.

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