作者: Kerry Claydon , Rahimah Awg Haji Tahir , Hajijah Mohd Said , Mahani Haji Lakim , Wanidawati Tamat
DOI: 10.1016/J.AQUACULTURE.2010.08.015
关键词: Infectious hypodermal and hematopoietic necrosis 、 Virology 、 Penaeus monodon 、 Virus 、 Specific-pathogen-free 、 White spot syndrome 、 Biology 、 Population 、 Prawn 、 Shrimp
摘要: Over a period of 30 months, wild black tiger shrimp (Penaeus monodon) were collected from the South China Sea in South-east Asia, an effort to establish specific pathogen free (SPF) stocks P. monodon Brunei Darussalam. The viral status broodstock was assessed by real-time and conventional polymerase chain reaction (PCR) using DNA reverse transcribed RNA extracted gill, hepatopancreas pleopods. Histopathology performed confirm PCR results. 270 collected, screened spawned. Of nine viruses assessed, infectious hypodermal hematopoietic necrosis virus (IHHNV) most commonly detected (19.6%), followed baculovirus (MBV) (7.4%), hepatopancreatic parvovirus (HPV) (3.8%), Mourilyan (MoV) (0.9%). only multiple infections found combination IHHNV MBV (2.2%). Interestingly, two virulent for monodon, white spot syndrome (WSSV) yellowhead (YHV) not any shrimp. It is concluded that waters have low prevalence pathogens, which has permitted development SPF as foundation population selective breeding.