Subcellular localization of Bic-D::GFP is linked to an asymmetric oocyte nucleus.

摘要: Bicaudal-D (Bic-D) is essential for the establishment of oocyte fate and subsequently polarity formation within developing Drosophila oocyte. To find out where in germ cells Bic-D performs its various functions we made transgenic flies expressing a chimeric Bic-D::GFP fusion protein. Once preferentially accumulates oocyte, it shows an initial anterior localization germarial region 2. In subsequent egg chamber stages 1–6 between nucleus posterior cortex focus that consistently aligned with crater-like indentation nucleus. After stage 6 fluorescent signal predominantly found dorso-anterior cortex. During different phases several genes have been to be required new distribution patterns. Dynein heavy chain (Dhc), spindle (spn) maelstrom (mael) are re-localization from position. Genes predicted encode proteins interact RNA (egalitarian orb) normal subcellular germarium, another potential binding protein, spn-E, proper transport nurse later oogenesis stages. The results indicate requires activity mRNA negative-end directed microtubule motor localize appropriate cellular domains. Asymmetric accumulation polarization may reflect function this machinery vectorial tethering defined by nuclear marks some first steps antero-posterior dorso-ventral formation.