Polar transport in the Drosophila oocyte requires Dynein and Kinesin I cooperation.

摘要: Abstract Background: The cytoskeleton and associated motors play an important role in the establishment of intracellular polarity. Microtubule-based transport is required many cell types for asymmetric localization mRNAs organelles. A striking example Drosophila oocyte, where microtubule-dependent processes govern positioning nucleus to distinct cortical domains that function as cytoplasmic determinants. conserved machinery mRNA nuclear involving Dynein has been postulated; however, precise plus- minus end-directed microtubule-based axis formation not yet understood. Results: Here, we show at mid-oogenesis depend on two motor proteins, Kinesin I. Both these microtubule cooperate polar bicoid gurken their respective domains. In contrast, I - mediated oskar posterior pole appears be independent Dynein. Beside roles RNA transport, both are involved exocytosis Gurken protein. Dynein-Dynactin complexes accumulate sites within oocyte: around a microtubule-independent manner through Kinesin-mediated transport. Conclusion: I, by driving opposing ends, concert establish polarity oocyte. Furthermore, Kinesin-dependent suggests components same complex therefore might recycling each other opposite pole.