Catharanthus roseus Aqueous Extract is Cytotoxic to Jurkat Leukaemic T-cells but Induces the Proliferation of Normal Peripheral Blood Mononuclear Cells.
作者： Ishak Mat , Nor Hazwani Ahmad , Rohanizah Abdul Rahim
DOI:
关键词: Catharanthus roseus 、 Cytotoxicity 、 Jurkat cells 、 Cytotoxic T cell 、 Molecular biology 、 Immunology 、 DNA fragmentation 、 Chemistry 、 Cell culture 、 Apoptosis 、 Peripheral blood mononuclear cell
摘要: Research on natural products has been widely used as a strategy to discover new drugs with potential for applications in complementary medicines because they have fewer side effects than conventional drugs. The aim of the present study was evaluate vitro cytotoxic crude aqueous Catharanthus roseus extract Jurkat cells and normal peripheral blood mononuclear (PBMCs). standardised vinblastine by high performance liquid chromatography (HPLC) determine cytotoxicity MTS [3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay. DNA fragmentation assay employed if cell death due apoptosis. results showed that induced at 24, 48 72 hours post-treatment time- dose-dependent manner. However, treated produced higher half maximal inhibitory concentration (IC50) values 2.55 μg/ml 2.38 μg/ml, respectively. In contrast, PBMC proliferation, especially after 24 treatment 1000 μg/ml. This result indicates C. differential inhibiting proliferation line promoting growth PBMCs. These data suggest may be applicable modulating transformed immune leukaemia patients.
cabdirect.org
usm.my 参考文章(21)
Wahl Sm, Mergenhagen Se, Mangan Df, Apoptosis in human monocytes: possible role in chronic inflammatory diseases. Journal of Periodontology. ,vol. 64, pp. 461- 466 ,(1993)
Shiv Pillai, Abul K. Abbas, Andrew H. Lichtman, Cellular and Molecular Immunology ,(1994)
Mariana Sottomayor, Alfonso Ros Barceló, The Vinca Alkaloids: From Biosynthesis and Accumulation in Plant Cells, to Uptake, Activity and Metabolism in Animal Cells Studies in natural products chemistry. ,vol. 33, pp. 813- 857 ,(2006) , 10.1016/S1572-5995(06)80041-4
Tatiana Okouneva, Leslie Wilson, Bridget T. Hill, Mary Ann Jordan, The Effects of Vinflunine, Vinorelbine, and Vinblastine on Centromere Dynamics1 Molecular Cancer Therapeutics. ,vol. 2, pp. 427- 436 ,(2003)
Siriporn Okonogi, Chadarat Duangrat, Songyot Anuchpreeda, Suganya Tachakittirungrod, Sombat Chowwanapoonpohn, Comparison of antioxidant capacities and cytotoxicities of certain fruit peels Food Chemistry. ,vol. 103, pp. 839- 846 ,(2007) , 10.1016/J.FOODCHEM.2006.09.034
V. Kuete, B. Ngameni, C.C. Fotso Simo, R. Kengap Tankeu, B. Tchaleu Ngadjui, J.J.M. Meyer, N. Lall, J.R. Kuiate, Antimicrobial activity of the crude extracts and compounds from Ficus chlamydocarpa and Ficus cordata (Moraceae) Journal of Ethnopharmacology. ,vol. 120, pp. 17- 24 ,(2008) , 10.1016/J.JEP.2008.07.026
Jean-Hugues Renault, Jean-Marc Nuzillard, Gaëlle Le Crouérour, Philippe Thépenier, Monique Zèches-Hanrot, Louisette Le Men-Olivier, Isolation of indole alkaloids from Catharanthus roseus by centrifugal partition chromatography in the pH-zone refining mode Journal of Chromatography A. ,vol. 849, pp. 421- 431 ,(1999) , 10.1016/S0021-9673(99)00495-1
King-Thom Chung, Tit Yee Wong, Cheng-I Wei, Yao-Wen Huang, Yuan Lin, Tannins and Human Health: A Review Critical Reviews in Food Science and Nutrition. ,vol. 38, pp. 421- 464 ,(1998) , 10.1080/10408699891274273
Weici Tang, Ingrid Hemm, Barbara Bertram, Recent development of antitumor agents from chinese herbal medicines; part I. Low molecular compounds. Planta Medica. ,vol. 69, pp. 97- 108 ,(2003) , 10.1055/S-2003-37718
Steve Hisiger, Mario Jolicoeur, Analysis of Catharanthus roseus alkaloids by HPLC Phytochemistry Reviews. ,vol. 6, pp. 207- 234 ,(2007) , 10.1007/S11101-006-9036-Y