Engineering designer beta cells with a CRISPR-Cas9 conjugation platform.
作者： Donghyun Lim , Vedagopuram Sreekanth , Kurt J. Cox , Benjamin K. Law , Bridget K. Wagner
DOI: 10.1038/S41467-020-17725-0
关键词: Cas9 、 Computational biology 、 Secretion 、 Insulin secretion 、 DNA 、 CRISPR 、 Protein domain 、 Oligonucleotide 、 Genome editing 、 Computer science
摘要: … Finally, the conjugation of ssODNs to Cas9 did not negatively affect cell proliferations, ensuring … Specifically, we successfully produced cells that cosecrete IL-10, a well-established anti-…
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