Differentiation of the Major Listeria monocytogenes Serovars by Multiplex PCR

作者: M. Doumith , C. Buchrieser , P. Glaser , C. Jacquet , P. Martin

DOI: 10.1128/JCM.42.8.3819-3822.2004

关键词: Invasive ListeriosisListeria monocytogenesPolymerase chain reactionPcr testSerotypeBiologyFood microbiologyMultiplex polymerase chain reactionMicrobiologyListeriaVirology

摘要: A new multiplex PCR assay was developed to separate the four major Listeria monocytogenes serovars isolated from food and patients (1/2a, 1/2b, 1/2c, 4b) into distinct groups. The test, which constitutes a rapid practical alternative laborious classical serotyping, successfully evaluated with 222 strains.

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