Single-strand conformation polymorphism (SSCP) analysis of Listeria monocytogenes iap gene as tool to detect different serogroups.

作者: Marisa Manzano , Luca Cocolin , Corrado Pipan , Elisabetta Falasca , Guiseppe A Botta

DOI: 10.1006/MCPR.1997.0137

关键词:

摘要: PCR-single-strand conformation polymorphism (PCR-SSCP) analysis is a convenient technique for the detection of mutations. As mobility single-stranded DNA sequence-dependent it could therefore be used to determine serotype-related sequence variations in Listeria monocytogenes. Sero-specific patterns were observed different L. monocytogenes serogroups.

参考文章(20)
M Louie, P Jayaratne, I Luchsinger, J Devenish, J Yao, W Schlech, A Simor, Comparison of ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for molecular typing of Listeria monocytogenes. Journal of Clinical Microbiology. ,vol. 34, pp. 15- 19 ,(1996) , 10.1128/JCM.34.1.15-19.1996
M L Gray, A H Killinger, Listeria monocytogenes and listeric infections. Bacteriological Reviews. ,vol. 30, pp. 309- 382 ,(1966) , 10.1128/BR.30.2.309-382.1966
Masato Orita, Takao Sekiya, Kenshi Hayashi, DNA sequence polymorphisms in Alu repeats Genomics. ,vol. 8, pp. 271- 278 ,(1990) , 10.1016/0888-7543(90)90282-Y
Masato Orita, Youichi Suzuki, Takao Sekiya, Kenshi Hayashi, Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction Genomics. ,vol. 5, pp. 874- 879 ,(1989) , 10.1016/0888-7543(89)90129-8
Marisa Manzano, Luca Cocolin, Carlo Cantoni, Giuseppe Comi, Detection and identification of Listeria monocytogenes from milk and cheese by a single-step PCR Molecular Biotechnology. ,vol. 7, pp. 85- 88 ,(1997) , 10.1007/BF02821546
Daniel B. Demers, Shannon J. Odelberg, L.McA. Fisher, Identification of a factor IX point mutation using SSCP analysis and direct sequencing. Nucleic Acids Research. ,vol. 18, pp. 5575- 5575 ,(1990) , 10.1093/NAR/18.18.5575
Leanne Cochrum, Kathleen S. Hruska, Edmund B. Rucker, Timothy C. Johnston, The nucleotide sequence of the luxD gene of Xenorhabdus luminescens Hm Nucleic Acids Research. ,vol. 18, pp. 5570- 5570 ,(1990) , 10.1093/NAR/18.18.5570
M. Orita, H. Iwahana, H. Kanazawa, K. Hayashi, T. Sekiya, Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 2766- 2770 ,(1989) , 10.1073/PNAS.86.8.2766