Microalgae-derived oxylipins decrease inflammatory mediators by regulating the subcellular location of NFκB and PPAR-γ

作者: Javier Ávila-Román , Elena Talero , Carolina de los Reyes , Sofía García-Mauriño , Virginia Motilva

DOI: 10.1016/J.PHRS.2017.10.009

关键词: Cell biologySignal transductionSubcellular localizationColitisColocalizationInflammationTumor necrosis factor alphaBiologyChemokineNFKB1Biochemistry

摘要: Oxylipins (OXLs) are bioactive molecules generated by the oxidation of fatty acids that promote resolution acute inflammation and prevent chronic inflammatory processes through molecular mechanisms not well known. We have previously reported anti-inflammatory activity microalgae-derived OXLs OXL-containing biomass in two bowel disease (IBD) models: 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis TNBS-induced recurrent colitis. In this study, we examined vitro mechanism action most abundant isolated from Chlamydomonas debaryana (13S-HOTE 13S-HODE) Nannochloropsis gaditana (15S-HEPE). These decreased IL-1β IL-6 pro-inflammatory cytokines production as iNOS COX-2 expression levels THP-1 macrophages. addition, IL-8 HT-29 colon cells, major chemokine produced these cells. The interaction with NFκB PPAR-γ signaling pathways was studied confocal microscopy. macrophages stimulated LPS TNFα respectively, a pre-treatment 13S-HOTE, 13S-HODE 15S-HEPE (100μM) resulted lower nuclear presence both cell lines. study subcellular localization showed treatment cells caused migration into nucleus. Colocalization analysis transcription factors LPS-stimulated or lowered colocalization similar to control value, increased cytosolic above level. results indicate could act agonist consequently inhibit pathway activation, thus lowering markers, highlighting therapeutic potential diseases such IBD.

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