Reduced in Vitro Production of Anti-Tetanus Toxoid Antibody after Repeated in Vivo Immunization with Tetanus Toxoid

摘要: Lymphocytes from individuals booster-immunized with soluble tetanus toxoid synthesized anti-tetanus antibodies in vitro the presence of pokeweed mitogen. The responsiveness initiated by day 14, peaked between days 20 and 40 declined 60 to 80. Although temporal kinetics antibody production was similar all individuals, maximum levels synthesis differed over an 8-fold range. quantity after experimental immunization correlated interval prior booster immunization. Thus, cultures B (0.4 × 10 6 ) T (1.6 lymphocytes receiving first ≥48 months up 170 units whereas reboosted within 4 a less than units. This correlation not observed total IgG. response appeared reflect vivo that showed greatest increase serum second later caused only slight antibody. decreased repeated suppressive activity radiosensitive but function non-T lymphocyte fraction. Removal phagocytic cells did individuals.