Polyethylenimine-mediated in vivo gene transfer of a transmembrane superantigen fusion construct inhibits B16 murine melanoma growth.

摘要: Immunotherapy has been proposed as a therapeutic strategy in advanced-stage melanomas which other options have little effect. The Staphylococcus enterotoxin A (SEA) used to stimulate an antitumoral immune response but its use is hampered by severe systemic side effects. Here, we show that SEA can be targeted melanoma cells limit these More specifically, nonviral vector, the cationic polymer, polyethylenimine (PEI), express transmembrane fusion construct (pSEA-TM) B16F10-induced subcutaneous mice. efficacy of this vivo transfection was enhanced concomitant infusion epinephrine induce local vasoconstriction. In conditions, repeated injections pSEA-TM/PEI complexes elicited significant response, evidenced tumor growth inhibition, without adverse T cell infiltration tumors, together with positive lymphocyte proliferation tests, suggested and responses. Altogether, PEI-mediated targeting efficiently stimulates antitumor inducing effects observed administration SEA.