Expression of KLF5 in odontoblastic differentiation of dental pulp cells during in vitro odontoblastic induction and in vivo dental repair.

摘要: Aim To identify whether Kruppel-like factor 5 (KLF5) was involved in odontoblastic differentiation during reparative dentine formation. Methodology Human Dental pulp cells (DPCs) were isolated from healthy human dental tissue and induced for differentiation. Alizarin Red staining, alkaline phosphatase (ALPase) activity, quantitative real-time PCR Western Blot performed to evaluate in vitro The expression profile of KLF5 the determined by Blot. Knock-down lentivirus-mediated shRNA determine function After direct capping with MTA, maxillary first molar segments dissected male Wistar rats prepared for histology analysis immunohistochemistry staining. Results Odontoblastic confirmed significantly increased (ALP; P = 0.004) activity upregulated differentiation-related genes including sialophosphoprotein (DSPP; matrix protein-1 (DMP-1; P = <0.001). cultured DPCs (P = 0.0002). knock-down impaired capping, bridge-like calcified tissues formed under perforation sites. expressed odontoblast-like beneath sites formation. Conclusions KLF5 might be process formation.