Quantitative Determination of High-temperature Requirement Protein A1 and Its Possible Associated Molecules during Induced Reparative Dentin Formation

摘要: Abstract Introduction The equilibrium of inhibitors and inducers plays an important role in the mineralization dentin during dentinogenesis. High-temperature requirement protein A1 (HtrA1) is a novel bone inhibitor involved physiological pathological formation. However, expression HtrA1 tooth unknown. purpose present study was to investigate localization quantity its possible related molecules induced reparative Methods Rats were randomly sacrificed after direct pulp capping on days 0, 7, 14, 21. Maxillary segments obtained routinely prepared for histological analysis, immunohistochemistry, quantum dots–based double immunofluorescence, CRi’s Nuance imaging system (CRI, Woburn, MA)–based quantitative determination. Results square measure values significantly increased day 7 continued increase until HtrA1, matrix Gla (MGP), nestin, sialoprotein positively stained colocalized odontoblasts and/or odontoblast-like cells zone expressions MGP enhanced did not change between Both correlated with dentin; however, no correlation found MGP. Conclusions could be observed might possibly process formation associated