Krüppel-like Factor 5 Transcription Factor Promotes Microsomal Prostaglandin E2 Synthase 1 Gene Transcription in Breast Cancer

摘要: The KLF5 (Kruppel-like factor 5) transcription is specifically expressed in a subset of estrogen receptor α-negative breast cancers. Although promotes cancer cell cycle progression, survival, and tumorigenesis, the mechanism by which still not entirely understood. Here, we demonstrate that mPGES1, encoding microsomal prostaglandin E2 synthase 1 (mPGES1), direct downstream target gene. overexpression or knockdown positively altered levels mPGES1 mRNA protein multiple lines. 12-O-Tetradecanoylphorbol-13-acetate induced expression both dosage- time-dependent manners. induction was essential for 12-O-tetradecanoylphorbol-13-acetate to induce expression. Additionally, bound gene proximal promoter activated its transcription. Both promoted production; regulated p21, p27, Survivin expression; likewise stimulated proliferation. Overexpression partially rescued knockdown-induced changes growth arrest MCF10A cells. Finally, correlated with α/progesterone receptor/HER2 triple-negative status. These findings suggest KLF5, making it new biomarker potential therapeutic