Endogenous Brain Pericytes Are Widely Activated and Contribute to Mouse Glioma Microvasculature

摘要: Glioblastoma multiforme (GBM) is the most common brain tumor in adults. It presents an extremely challenging clinical problem, and treatment very frequently fails due to infiltrative growth, facilitated by extensive angiogenesis neovascularization. Pericytes constitute important part of GBM microvasculature. The contribution endogenous pericytes vasculature is, however, unclear. In this study, we determine site activation extent vasculature. GL261 mouse glioma was orthotopically implanted mice expressing green fluorescent protein (GFP) under pericyte marker regulator G signaling 5 (RGS5). Host were not only activated within glioma, but also cortical areas overlying tumor, ipsilateral subventricular zone hemisphere contralateral tumor. host-derived that infiltrated mainly localized vessel wall. Infiltrating GFP positive co-expressed markers platelet-derived growth factor receptor-β (PDGFR-β) neuron-glial antigen 2. Interestingly, more than half all PDGFR-β contributed host brain. We did find any evidence RGS5 adopt another phenotype paradigm. conclude become widespread response orthotopic glioma. are recruited into a major population.