Chapter V Electron Microscopy of Small Particles, Macromolecular Structures and Nucleic Acids

摘要: Publisher Summary This chapter presents the electron microscopy of small particles, macromolecular structures, and nucleic acids. The resolving power current production transmission microscopes is about 0.2 nm as evidenced by measurements on lattice patterns obtained with inorganic crystalline specimens. On other hand, resolution that can be demonstrated in biological specimens 2 or one order magnitude poorer. negative staining contrast method has been successfully applied to an enormous variety structures such viruses all types, bacterial cells their appendages, flagellae pili, membranes many varieties protein molecules both individually ordered arrays either naturally occurring, collagen, laboratory produced crystals. To obtain a useful image negatively stained preparation specimen contain at least proportion lipid. Nucleic acids when stripped protein, do not give good images methods. central importance biology resulted much effort being spent preparing would yield meaningful microscope images. In addition “descriptive” studies have made Kleinschmidt techniques there steady development methods whereby location specific chemical functional sites visualized. describes most successful now use for Kleinschmidt-type together variations, which proved special instances.