Directional genomic hybridization: inversions as a potential biodosimeter for retrospective radiation exposure

摘要: Chromosome aberrations in blood lymphocytes provide a useful measure of past exposure to ionizing radiation. Despite the widespread and successful use dicentric assay for retrospective biodosimetry, approach suffers substantial drawbacks, including fact that dicentrics circulating have rather short half-life (roughly 1–2 years by most estimates). So-called symmetrical such as translocations are far more stable regard, but their high background frequency, which increases with age, also makes them less than ideal biodosimetry. We developed cytogenetic potential biodosimetry is based on detection chromosomal inversions, another aberration whose transmissibility (stability) ostensibly high. Many well-known difficulties associated inversion were circumvented through directional genomic hybridization, method molecular cytogenetics labor intensive better able detect small inversions other currently available approaches. Here, we report dose-dependent induction following radiations vastly different ionization densities [i.e., linear energy transfer (LET)]. Our results show dramatic difference yields induced low-LET gamma rays, compared damaging high-LET charged particles similar those encountered deep space.