On-site alginate gelation for enhanced cell proliferation and uniform distribution in porous scaffolds.

摘要: High cell density and uniformity in a tissue-engineered construct is essential to expedite the formation of uniform extracellular matrix. In this study, we demonstrated an on-site gelation approach increase cellular population through porous scaffolds using alginate as gelling material. The was triggered during seeding shown effectively restrain cells scaffold subsequent cultivation. initial demonstration effectiveness system made with chondrocyte cells, targeted at functional restoration damaged or dysfunctional cartilage. By limiting mobility, increased by 89% after 7 days culture encapsulating gel opposed 36% without gel. distribution throughout gelled found be more than nongelled scaffold. SEM analysis revealed that exhibited typical chondrocytic morphology. Improved functionality verified low levels collagen type I gene expression steady activity II over 3 weeks Alternatively, seeded conventional cell-seeding method expression, indicating possibility dedifferentiation long-term culture. Success chitosan-alginate model suggested flexible could potentially applied other tissue types for enhanced engineering development.