Generation of induced pluripotent stem cells without genetic defects by small molecules
作者： Hang-Soo Park , Insik Hwang , Kyung-Ah Choi , Hyesun Jeong , Ji-Yun Lee
DOI: 10.1016/J.BIOMATERIALS.2014.10.055
关键词: Reprogramming 、 Epigenetics 、 Cell biology 、 Induced pluripotent stem cell 、 Gene 、 Cell growth 、 Biology 、 Molecular biology 、 Ascorbic acid 、 Germ layer 、 Genome instability
摘要: The generation of induced pluripotent stem cells (iPSCs) often causes genetic and epigenetic defects, which may limit their clinical applications. Here, we show that reprogramming in the presence small molecules preserved genomic stability iPSCs by inhibiting DNA double-strand breaks (DSBs) activating Zscan4 gene. Surprisingly, protected normal karyotype facilitating repair DSBs occurred during early process long-term culture iPSCs. stemness cell growth iPSCs(+) were normally sustained with high expression pluripotency genes compared iPSCs(-). Moreover, maintained differentiation potential for three germ layers, whereas it was lost Our results demonstrate defined are potent factors quality preservation integrity process.
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