Molecular detection, phylogenetic analysis, and identification of transcription motifs in feline leukemia virus from naturally infected cats in malaysia.
作者： Faruku Bande , Siti Suri Arshad , Latiffah Hassan , Zunita Zakaria
DOI: 10.1155/2014/760961
关键词: Phylogenetic tree 、 Nested polymerase chain reaction 、 Transcription (biology) 、 Provirus 、 Virology 、 Biology 、 Enhancer 、 DNA 、 Homology (biology) 、 Feline leukemia virus
摘要: A nested PCR assay was used to determine the viral RNA and proviral DNA status of naturally infected cats. Selected samples that were FeLV-positive by subjected sequencing, phylogenetic analysis, motifs search. Of 39 positive for FeLV p27 antigen, 87.2% (34/39) confirmed with PCR. detected in 38 (97.3%) p27-antigen negative samples. Malaysian isolates are found be highly similar a homology 91% 100%. Phylogenetic analysis revealed divided into two clusters, majority (86.2%) sharing similarity FeLV-K01803 fewer (13.8%) FeLV-GM1 strain. Different enhancer including NF-GMa, Krox-20/WT1I-del2, BAF1, AP-2, TBP, TFIIF-beta, TRF, TFIID occur either single, duplicate, triplicate, or sets 5 different positions within U3-LTR-gag region. The present result confirms occurrence provirus similar, them closely related UK isolate. This study provides first molecular based information on Malaysia. Additionally, likely associated pathogenesis have been identified.
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