Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene.
作者： T. Velu , L Beguinot , W. Vass , M. Willingham , G. Merlino
关键词:
摘要: The epidermal growth factor (EGF) receptor gene EGFR has been placed in a retrovirus vector to examine the properties of cells that experimentally overproduce full-length EGF receptor. NIH 3T3 transfected with viral DNA or infected corresponding rescued developed fully transformed phenotype vitro required both functional expression and presence medium. Cells expressing 4 x 10(5) receptors formed tumors nude mice, while control did not. Therefore, retrovirus, which had titer on was greater than 10(7) focus-forming units per milliliter, can efficiently transfer express this gene, increased numbers contribute phenotype.
sciencemag.org
harvard.edu参考文章(26)
M C Willingham, N Richert, L Beguinot, D Werth, I Pastan, S Ito, Functional studies on the EGF receptor with an antibody that recognizes the intracellular portion of the receptor. Journal of Biological Chemistry. ,vol. 261, pp. 1801- 1807 ,(1986) , 10.1016/S0021-9258(17)36011-8
A. Bernstein, Robin Weiss, RNA tumor viruses University of California, San Francisco. Archives and Special Collections. Harold E. Varmus Papers. ,(1982)
D R Lowy, E Rands, E M Scolnick, Helper-independent transformation by unintegrated Harvey sarcoma virus DNA. Journal of Virology. ,vol. 26, pp. 291- 298 ,(1978) , 10.1128/JVI.26.2.291-298.1978
F.L. Graham, A.J. van der Eb, A new technique for the assay of infectivity of human adenovirus 5 DNA Virology. ,vol. 52, pp. 456- 467 ,(1973) , 10.1016/0042-6822(73)90341-3
Martine F. Roussel, Thomas J. Dull, Carl W. Rettenmier, Peter Ralph, Axel Ullrich, Charles J. Sherr, Transforming potential of the c-fms proto-oncogene (CSF-1 receptor) Nature. ,vol. 325, pp. 549- 552 ,(1987) , 10.1038/325549A0
Towia A. Libermann, Harris R. Nusbaum, Nissim Razon, Richard Kris, Irit Lax, Hermona Soreq, Nigel Whittle, Michael D. Waterfield, Axel Ullrich, Joseph Schlessinger, Amplification, enhanced expression and possible rearrangement of EGF receptor gene in primary human brain tumours of glial origin Nature. ,vol. 313, pp. 144- 147 ,(1985) , 10.1038/313144A0
C. Lin, W. Chen, W Kruiger, L. Stolarsky, W Weber, R. Evans, I. Verma, G. Gill, M. Rosenfeld, Expression cloning of human EGF receptor complementary DNA: gene amplification and three related messenger RNA products in A431 cells Science. ,vol. 224, pp. 843- 848 ,(1984) , 10.1126/SCIENCE.6326261
C.Richter King, Matthias H. Kraus, Lewis T. Williams, Glenn T. Merlino, Ira H. Pastan, Stuart A. Aaronson, Human tumor cell lines with EGF receptor gene amplification in the absence of aberrant sized mRNAs Nucleic Acids Research. ,vol. 13, pp. 8477- 8486 ,(1985) , 10.1093/NAR/13.23.8477
O Tsutsumi, H Kurachi, T Oka, A Physiological Role of Epidermal Growth Factor in Male Reproductive Function Science. ,vol. 233, pp. 975- 977 ,(1986) , 10.1126/SCIENCE.3090686
A. Ullrich, L. Coussens, J. S. Hayflick, T. J. Dull, A. Gray, A. W. Tam, J. Lee, Y. Yarden, T. A. Libermann, J. Schlessinger, J. Downward, E. L. V. Mayes, N. Whittle, M. D. Waterfield, P. H. Seeburg, Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells Nature. ,vol. 309, pp. 418- 425 ,(1984) , 10.1038/309418A0