Expression cloning of human EGF receptor complementary DNA: gene amplification and three related messenger RNA products in A431 cells
作者： C. Lin , W. Chen , W Kruiger , L. Stolarsky , W Weber
关键词:
摘要: In order to further define the mechanisms by which polypeptide growth factors regulate gene transcription and cellular growth, expression cloning techniques were used select human epidermal factor (EGF) receptor complementary DNA clones. The EGF 3' coding domain shows striking homology transforming product of avian erythroblastosis virus (v-erbB). Over-expression receptors in A431 cell lines correlates with increased mRNA levels amplification (up 110 times) apparently singular gene. There appear be three cytoplasmic polyadenylated RNA products cells, one contains only 5' (EGF binding domain) sequences is postulated encode secreted receptor-related protein.
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G.M. Wahl, R.A. Padgett, G.R. Stark, Gene amplification causes overproduction of the first three enzymes of UMP synthesis in N-(phosphonacetyl)-L-aspartate-resistant hamster cells. Journal of Biological Chemistry. ,vol. 254, pp. 8679- 8689 ,(1979) , 10.1016/S0021-9258(19)86945-4
S Iwashita, C F Fox, Epidermal growth factor and potent phorbol tumor promoters induce epidermal growth factor receptor phosphorylation in a similar but distinctively different manner in human epidermoid carcinoma A431 cells. Journal of Biological Chemistry. ,vol. 259, pp. 2559- 2567 ,(1984) , 10.1016/S0021-9258(17)43390-4
G Carpenter, KJ Lembach, MM Morrison, S Cohen, Characterization of the binding of 125-I-labeled epidermal growth factor to human fibroblasts. Journal of Biological Chemistry. ,vol. 250, pp. 4297- 4304 ,(1975) , 10.1016/S0021-9258(19)41417-8
Stanley Cohen, Isolation of a Mouse Submaxillary Gland Protein Accelerating Incisor Eruption and Eyelid Opening in the New-born Animal Journal of Biological Chemistry. ,vol. 237, pp. 1555- 1562 ,(1962) , 10.1016/S0021-9258(19)83739-0
H. Ushiro, S. Cohen, Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes. Journal of Biological Chemistry. ,vol. 255, pp. 8363- 8365 ,(1980) , 10.1016/S0021-9258(18)43497-7
C Cochet, G N Gill, J Meisenhelder, J A Cooper, T Hunter, C-kinase phosphorylates the epidermal growth factor receptor and reduces its epidermal growth factor-stimulated tyrosine protein kinase activity. Journal of Biological Chemistry. ,vol. 259, pp. 2553- 2558 ,(1984) , 10.1016/S0021-9258(17)43389-8
Allan M. Maxam, Walter Gilbert, [57] Sequencing end-labeled DNA with base-specific chemical cleavages Nucleic Acids Part I. ,vol. 65, pp. 499- 560 ,(1980) , 10.1016/S0076-6879(80)65059-9
S. Cohen, H. Ushiro, C. Stoscheck, M. Chinkers, A native 170,000 epidermal growth factor receptor-kinase complex from shed plasma membrane vesicles. Journal of Biological Chemistry. ,vol. 257, pp. 1523- 1531 ,(1982) , 10.1016/S0021-9258(19)68224-4
Ueli Schibler, Toni Wyler, Otto Hagenbüchle, Changes in size and secondary structure of the ribosomal transcription unit during vertebrate evolution Journal of Molecular Biology. ,vol. 94, pp. 503- 517 ,(1975) , 10.1016/0022-2836(75)90217-X
M. D. Hollenberg, P. Cuatrecasas, Epidermal Growth Factor: Receptors in Human Fibroblasts and Modulation of Action by Cholera Toxin Proceedings of the National Academy of Sciences of the United States of America. ,vol. 70, pp. 2964- 2968 ,(1973) , 10.1073/PNAS.70.10.2964