Circulating tumor DNA detectable in early- and late-stage colorectal cancer patients.
作者： Ying-Chi Yang , Dong Wang , Lan Jin , Hong-Wei Yao , Jing-Hui Zhang
DOI: 10.1042/BSR20180322
关键词: Biopsy 、 Disease 、 Internal medicine 、 Medicine 、 Mutation 、 Liquid biopsy 、 Colorectal cancer 、 Gene mutation 、 Cancer 、 Oncology 、 Biomarker (medicine)
摘要: Characterization, diagnosis, and treatment of colorectal cancers (CRC) is difficult due to limited biopsy information, impracticality repeated biopsies, cancer biomarker fallibility. Circulating tumor DNA (ctDNA) has recently been investigated as a non-invasive way gain representative gene mutations in tumors, addition monitoring disease progression response treatment. We analyzed ctDNA concentrations 47 early- late-stage CRC patients using targetted sequencing approach panel that covers 50 cancer-related genes. 37 genes were identified 93.6% the (n=47). The results showed TP53, PIK3CA, APC, EGFR most frequently mutated Stage IV had significantly higher concentration than I patients, increased correlated with size. Additionally, detection was found be greater predictor when compared five known commonly used biomarkers. present study supports use liquid clinical information may facilitate early diagnosis improve patient prognosis.
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