Correlation between MBD2 Expressions in Colorectal Cancer Tissue and Blood
作者： Hyun-Koun Park , Jeong-Eun Lee , Ryung-Ah Lee , Kwang Ho Kim
DOI: 10.3393/JKSC.2008.24.6.447
关键词: DNA demethylation 、 DNA methylation 、 Cancer 、 Whole blood 、 Carcinogenesis 、 Gene expression 、 Pathology 、 Andrology 、 Colorectal cancer 、 CpG site 、 Medicine
摘要: nism for modification of genetic expression without a change in the DNA sequence. MBD2 (methyl-CpG-binding domain 2 protein) belongs to family enzymes concerning demethylation and suppresses hypermethylation CpG island transcription. In this study, we investigated blood tissue colorectal cancer patients compared two levels. Methods: The 68 included study were with who had undergone surgery at our hospital, 50 other no malignant disease recruited from normal populations. Total RNA samples isolated whole tissues specimens using TRI REAGENT BD kit. MBD was measured by real-time quantitative reverse transcription-polymerase chain reaction assays. Results: mean age older case group than control group. level not different between groups. group, lower under all conditions, that difference statistically significant (P＜0.01). showed negative correlation patients, coefficient R=0.073, but result (P=0.611). Conclusions: same significantly higher expression. suggests may affect mechanism carcinogenesis.
koreascience.or.kr参考文章(18)
C Müller-Tidow, K Kügler, S Diederichs, S Klümpen, M Möller, U Vogt, R Metzger, P M Schneider, W E Berdel, H Serve, Loss of expression of HDAC-recruiting methyl-CpG-binding domain proteins in human cancer. British Journal of Cancer. ,vol. 85, pp. 1168- 1174 ,(2001) , 10.1054/BJOC.2001.2041
Peter A. Jones, Stephen B. Baylin, The fundamental role of epigenetic events in cancer Nature Reviews Genetics. ,vol. 3, pp. 415- 428 ,(2002) , 10.1038/NRG816
Yong Zhu, Margaret R. Spitz, Hui Zhang, H. Barton Grossman, Marsha L. Frazier, Xifeng Wu, Methyl-CpG-binding domain 2: a protective role in bladder carcinoma. Cancer. ,vol. 100, pp. 1853- 1858 ,(2004) , 10.1002/CNCR.20199
Joe D. Lewis, Richard R. Meehan, William J. Henzel, Ingrid Maurer-Fogy, Peter Jeppesen, Franz Klein, Adrian Bird, Purification, sequence, and cellular localization of a novel chromosomal protein that binds to Methylated DNA Cell. ,vol. 69, pp. 905- 914 ,(1992) , 10.1016/0092-8674(92)90610-O
Carolyn Bariol, Catherine Suter, Kay Cheong, Su-Lyn Ku, Alan Meagher, Nicholas Hawkins, Robyn Ward, None, The relationship between hypomethylation and CpG island methylation in colorectal neoplasia. American Journal of Pathology. ,vol. 162, pp. 1361- 1371 ,(2003) , 10.1016/S0002-9440(10)63932-6
Andrew Slack, Veronica Bovenzi, Pascal Bigey, M. A. Ivanov, Shyam Ramchandani, Sanjoy Bhattacharya, Benjamin tenOever, B. Lamrihi, Daniel Scherman, Moshe Szyf, AntisenseMBD2 gene therapy inhibits tumorigenesis The Journal of Gene Medicine. ,vol. 4, pp. 381- 389 ,(2002) , 10.1002/JGM.288
Manel Esteller, James G. Herman, Cancer as an epigenetic disease: DNA methylation and chromatin alterations in human tumours. The Journal of Pathology. ,vol. 196, pp. 1- 7 ,(2002) , 10.1002/PATH.1024
HEE CHEOL KIM, SUN AE ROH, IN HWA GA, JUNG-SUN KIM, CHANG SIK YU, JIN CHEON KIM, CpG island methylation as an early event during adenoma progression in carcinogenesis of sporadic colorectal cancer. Journal of Gastroenterology and Hepatology. ,vol. 20, pp. 1920- 1926 ,(2005) , 10.1111/J.1440-1746.2005.03943.X
M Hattori, H Sakamoto, K Satoh, T Yamamoto, DNA demethylase is expressed in ovarian cancers and the expression correlates with demethylation of CpG sites in the promoter region of c-erbB-2 and survivin genes. Cancer Letters. ,vol. 169, pp. 155- 164 ,(2001) , 10.1016/S0304-3835(01)00499-2
Jiang-Cheng Shen, William M. Rideout, Peter A. Jones, High frequency mutagenesis by a DNA methyltransferase. Cell. ,vol. 71, pp. 1073- 1080 ,(1992) , 10.1016/S0092-8674(05)80057-1