Loss of expression of HDAC-recruiting methyl-CpG-binding domain proteins in human cancer.

摘要: Dysregulation of CpG-methylation is a common feature many human cancers and tumour suppressor genes can be silenced by hypermethylation. Recently, 2 methyl-CpG-binding domain proteins have been linked to gene inactivation their ability recruit co-repressors HDAC-activity methylated promoters. Here, we analysed mRNA expression these genes, MeCP2 MBD2, in wide variety primary tumours. In solid tumours, levels MBD2 (57/71) (64/71) were significantly reduced the majority tumours as detected quantitative real-time RT-PCR. Western blot analyses matched tumour–normal samples patients with non-small-cell lung cancer (NSCLC) indicated protein significant percentage patients. acute myelogenous leukaemia (n = 26), only slightly did not differ between at diagnosis or time relapse. early-stage NSCLC 70) was lower squamous cell carcinoma than adenocarcinoma large (P 0.03 P 0.01). To further elucidate mechanisms regulation, regulation during haematopoietic differentiation. No changes found when NB4 cells differentiated toward granulocytes suggesting that neither differentiation nor cycle status relevant for cancer. conclusion, loss suggests potential role this phenomenon development © 2001 Cancer Research Campaign http://www.bjcancer.com