TGF-β superfamily regulates a switch that mediates differentiation either into adipocytes or myocytes in left atrium derived pluripotent cells (LA-PCS)
作者： Nanako Kawaguchi , Ryota Nakao , Makoto Yamaguchi , Daisuke Ogawa , Rumiko Matsuoka
DOI: 10.1016/J.BBRC.2010.04.123
关键词: Stem cell 、 Myogenesis 、 Biology 、 Endocrinology 、 Internal medicine 、 Cellular differentiation 、 Cell type 、 Cell fate determination 、 Adipogenesis 、 Myocyte 、 Signal transduction 、 Cell biology
摘要: Many stem cell studies have focused on the subject of fate and signal molecules that modulate regulatory switches for a given differentiation pathway. Genome-wide screens determination signals require source differentiates purely into single type. From adult rat left atrium, we established LA-PCs cardiac/skeletal myocytes or adipocytes with almost 100% purity. In this study, compared gene expression profiles undifferentiated those differentiated cells [adipocytes (Adi) (Myo)] to identify set switch adipocyte myocyte differentiation. Microarray analysis verified feasibility genome-wide screening by method. Using pathway screen, found members TGF-beta superfamily transduction pathways adipocyte/myocyte switch. Further determined recombinant inhibits adipogenesis induces myogenesis simultaneously in dose-dependent manner. Moreover, noggin fully developed beating cardiac vitro. These results provided new insight validated method their identification.
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