O6-methylguanine-DNA methyltransferase gene (MGMT) expression in human glioblastomas in relation to patient characteristics and p53 accumulation

摘要: Repair of cytotoxic DNA damage by O6-methylguanine-DNA methyltransferase (MGMT) is a potentially important factor chemoresistance to chloroethylnitrosoureas (CENUs), commonly used in the treatment glioblastoma multiforme (GBM). The value p53 as prognostic GBMs remains unclear, but possible relationship between MGMT gene expression and has been suggested. To further examine these GBM characteristics vivo, we assessed using semi-quantitative RT-PCR alteration immuno-histochemistry on series 39 GBMs. was inversely correlated with age (p < 0.03), consistent results others. Interestingly, tumors from male patients had higher mRNA amounts than female 0.03). No implication observed either for or accumulation. However, significantly lower p53-altered GBM, regardless percentage positive cells 0.01). Our observation suggests that human glial tumors, low level might promote alteration, probably via mutation its gene. Int. J. Cancer (Pred. Oncol.) 84:416–420, 1999. © 1999 Wiley-Liss, Inc.