MDM2 and p53 expression in gliomas: a multivariate survival analysis including proliferation markers and epidermal growth factor receptor

摘要: p53 and the murine double minute 2 (MDM2) oncoprotein expression was evaluated in paraffin-embedded tissue from 61 patients with central nervous system gliomas (53 astrocytomas eight oligodendrogliomas) related to proliferation-associated markers [i.e. proliferating cell nuclear antigen (PCNA), Ki-67 organizer regions (NORs)] epidermal growth factor receptor (EGFR). We used monoclonal antibodies PC-10, MIB-1, DO-1, 1B1O EGFR 113 colloid silver nitrate (AgNOR) technique. MDM2 were co-expressed 28% of cases. A p53-positive/MDM2-negative phenotype observed 15% a p53-negative/MDM2-positive 20% There positive correlation grade proliferation indices. Univariate analysis group diffuse showed that older age, high histological grade, PCNA labelling index (LI) AgNOR score associated reduced overall survival (P < 0.05). LI, score, tumour location influenced disease-free 0.05), whereas only parameters affecting post-relapse LI 0.1). Multivariate revealed radiotherapy, independent predictors survival. type therapy survival, predictor Our results indicate as well (PCNA Ki-67) are useful indicators astrocytoma patients.