Human gastrin-releasing peptide triggers growth of HepG2 cells through blocking endoplasmic reticulum stress-mediated apoptosis.

摘要: Gastrin-releasing peptide (GRP) is a kind of neural that plays an important role in the growth various human cancer cells. However, very little known about relationship between GRP and apoptosis hepatocellular carcinoma This study investigated influences on apoptosis, as well mechanism triggers HepG2 growth. The effects cell proliferation were examined by analysis lactate dehydrogenase. GRP, caspase 12, CHOP protein detected HL-7702 cells Western blot, endoplasmic reticulum (ER) stress-related mRNA transcription was reverse polymerase chain reaction. To explore specific pathway which induces growth, we apoptosis-related pathway. expression inhibited tunicamycin triggered ER stress-associated XBP1, ATF4, TRAF2 transcription. Three main stress-unfolded response pathways proteins, including spliced cleaved ATF6, IRE1-α, PERK, eIF2-α, increased significantly. Furthermore, 12 activation blocked when expressed either or In conclusion, through blocking stress-mediated