Improved burn wound healing by the antimicrobial peptide LLKKK18 released from conjugates with dextrin embedded in a Carbopol gel

摘要: Abstract Antimicrobial peptides (AMPs) are good candidates to treat burn wounds, a major cause of morbidity, impaired life quality and resources consumption in developed countries. We took advantage commercially available hydrogel, Carbopol®, vehicle for topical administration that maintains moist environment within the wound site. hypothesized incorporation LLKKK18 conjugated dextrin would improve healing process rat burns. Whereas hydrogel improves healing, released from conjugates further accelerated closure, simultaneously improving healing. Indeed, release reduced oxidative stress inflammation (low neutrophil macrophage infiltration pro-inflammatory cytokines levels). Importantly, it induced faster resolution inflammatory stage through early M2 recruitment. In addition, stimulated angiogenesis (increased VEGF microvessel development vivo ). Moreover, collagen staining evaluated by Masson’s Trichrome was visually much more intense after treatment with LLKKK18, suggesting higher deposition. Overall, we generated an effective, safe inexpensive formulation wound, easy apply remove, potential prevent infection due presence antimicrobial peptide. These findings propel us study this LLKKK18-containing formulation, setting foundations towards therapeutic approach treatment. Statement Significance This work presents newly holds great as It is based on sustained delivery peptide – dextrin, degradation backbone upon exposure α-amylases. Conjugates were embedded suitable provides wound. obtained efficient, non-expensive apply-and-remove, has first time ability demonstrated its main mechanisms action identified.