Callus and Shoot Regeneration from Protoplasts of Proso Millet (Panicum miliaceum L.)

摘要: Summary A system for the regeneration of callus and albino plantlets from protoplasts proso millet is described. Rapidly growing suspensions derived mature seeds were used as source protoplasts. Protoplasts cultured in drops 0.07–0.25 ml KM medium containing 1.0 mg · l−1 2,4-D BAP. After 5 days, 7% had reformed cell walls divided to form microcalli, but only 0.4% plated formed macrocalli. Over 20 shoots, plantlets, many roots initiated by apparent somatic embryogenesis protoplast-derived calli one or more passages on LS minus hormones.