Altered expression of epidermal growth factor receptor gene in a classical multidrug-resistant variant of a human cancer cell line, KB.
作者： Hiroshi Takano , Kimitoshi Kohno , Norio Shiraishi , Shin-ichi Sato , Kuni-ichi Asoh
DOI: 10.1111/J.1349-7006.1989.TB02322.X
关键词: Epidermal growth factor receptor 、 Exotoxin 、 Growth factor 、 Cell culture 、 Biology 、 Genetics 、 Clone (B-cell biology) 、 Molecular biology 、 Epidermal growth factor 、 Northern blot 、 In vitro
摘要: Abstract A variant clone resistant to high doses of colchicine (KB-C1) derived from human cancer KB cell line is various anticancer agents. The KB-C1 cells were much more epidermal growth factor and a chimeric toxin, EGF-Pseudomonas exotoxin (PE), than the parental cells. have decreased numbers EGF-receptors, though affinity receptors similar that in drug-sensitive revertant (C1-R2) partially recovered its EGF-receptor activity. Northern blot analysis showed level mRNA cells, while multidrug-resistance gene, mdr-1, was expressed at very levels but not or C1-R2 drug-resistant less tumorigenic when injected into nude mice. expression these may be one pleiotropic properties multidrug-resistant perhaps represent basis for their reduced tumorigenicity.
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