The evaluation of forty-three plant species for in vitro antimycobacterial activities ; isolation of active constituents from Psoralea corylifolia and Sanguinaria canadensis

摘要: Extracts from forty-three plant species were selected on account of reported traditional uses for the treatment TB and/or leprosy. These assayed antimycobacterial activities. A simple in vitro screening assay was employed using two model mycobacteria, M. aurum and smegmatis. Crude methanolic extracts three plants, C. mukul, P. corylifolia S. canadensis, found to have significant activity against only (MIC=62.5 microg/ml). Bioassay guided fractionation led isolation known benzophenanthridine alkaloids, sanguinarine (1) chelerythrine (2), roots canadensis phenolic meroterpene, bakuchiol (3) seeds corylifolia. The resin mukul lead a decrease hence further work not pursued. Compound (2) most active smegmatis (IC(50)=7.30 microg/ml [19.02 microM] 29.0 [75.56 microM], respectively). susceptible organism all compounds. No difference observed when alkaloids tested media differing pH values. activities pure compounds comparable with those bovis BCG compound being (M. BCG, IC(50)=14.3 [37.3 microM]). results support use these plants medicine.