Identification and validation of prognostic markers in breast cancer with the complementary use of array-CGH and tissue microarrays.

摘要: Gene amplification, an important mechanism of oncogene activation in breast cancer, can have both prognostic and therapeutic implications. In this work, attempt is made to identify amplified genes that be used improve prognostication cancer. A series 52 node-negative tumours was screened for genomic gains at 57 loci by array-CGH. subset these identified could divide the into two divergent outcome groups either long-term survivors or early disease-related deaths (p = 0.01) using a combination k-means clustering statistical analysis. The significance amplification four (EMS1, TOP2A, CCNE1, ERBB2) then evaluated, fluorescent situ hybridization on tissue microarray, second larger 'validation' 232 with median follow-up 4.8 years. Adverse associated TOP2A 0.004); ERBB2 0.002); combined ERBB2, EMS1 0.01). more common (26% cases) than previously reported but, isolation, had no significance. Amplification seen only 6% cases, role. These results indicate complementary use array-CGH microarrays has potential help identification validation molecular markers classify cancers different groups.