Macroscale versus microscale methods for physiological analysis of biofilms formed in 96-well microtiter plates

摘要: Microtiter plates with 96 wells have become one of the preferred platforms for biofilm studies mainly because they enable high-throughput assays. In this work, macroscale and microscale methods were used to study impact hydrodynamic conditions on physiology location Escherichia coli JM109(DE3) biofilms formed in microtiter plates. Biofilms shaking static conditions, two parameters assayed: total amount was measured by crystal violet assay metabolic activity determined resazurin assay. From point view, there no statistically significant differences between conditions. However, at a level, both revealed using scanning electron microscopy (SEM). It observed that morphology spatial distribution along wall different these Simulation inside performed computational fluid dynamics (CFD). These simulations showed shear strain rate unevenly distributed walls during regions higher obtained closer air/liquid interface. Additionally, it shown subjected rates associated formation containing cells smaller size. Conversely, lower prone more uniform adhered larger The results presented work highlight wealth information may be gathered complementing approaches analysis experiments.