How to Study Biofilms after Microbial Colonization of Materials Used in Orthopaedic Implants.

摘要: Over the years, various techniques have been proposed for quantitative evaluation of microbial biofilms. Spectrophotometry after crystal violet staining is a widespread method biofilm evaluation, but several data indicate that it does not guarantee good specificity, although rather easy to use and cost saving. Confocal laser microscopy one most sensitive specific tools study biofilms, largely used research. However, in some cases, no measurement matrix thickness or amount embedded microorganisms has performed, due limitation availability dedicated software. For this reason, we developed protocol evaluate formed on sandblasted titanium orthopaedic implants, allows biomass volume included cells. Results reproducibility terms Moreover, proved be applicable efficacy different anti-biofilm treatments setting. Summing up, here described valid inexpensive prosthetic implant materials.