The Expression Profile of Phosphatidylinositol in High Spatial Resolution Imaging Mass Spectrometry as a Potential Biomarker for Prostate Cancer
作者： Takayuki Goto , Naoki Terada , Takahiro Inoue , Kenji Nakayama , Yoshiyuki Okada
DOI: 10.1371/JOURNAL.PONE.0090242
关键词: Cancer research 、 Biomarker (medicine) 、 Epithelium 、 Prostate cancer 、 Prostatectomy 、 Chemistry 、 Tandem mass spectrometry 、 Pathology 、 Cancer 、 Mass spectrometry imaging 、 Prostate 、 General Biochemistry, Genetics and Molecular Biology 、 General Agricultural and Biological Sciences 、 General Medicine
摘要: High-resolution matrix-assisted laser desorption/ionization imaging mass spectrometry (HR-MALDI-IMS) is an emerging application for the comprehensive and detailed analysis of spatial distribution ionized molecules in situ on tissue slides. HR-MALDI-IMS negative mode a range m/z 500–1000 was performed optimal cutting temperature (OCT) compound-embedded human prostate samples obtained from patients with cancer at time radical prostatectomy. 14 discovery set identified 26 as highly expressed prostate. Tandem (MS/MS) showed that these included phosphatidylinositols (PIs), 3 phosphatidylethanolamines (PEs) phosphatidic acids (PAs). Among PIs, expression PI(18:0/18:1), PI(18:0/20:3) PI(18:0/20:2) were significantly higher than benign epithelium. A biomarker algorithm formulated by analyzing profiles PIs epithelium using orthogonal partial least squares discriminant (OPLS-DA). The sensitivity specificity this diagnosis 24 validation 87.5 91.7%, respectively. In conclusion, several being more These differences PI may serve novel diagnostic tool cancer.
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