Identification of gel-separated tumor marker proteins by mass spectrometry

作者: Ann-Charlotte Bergman , Timothy Benjamin , Ayodele Alaiya , Mark Waltham , Kazuyazu Sakaguchi

DOI: 10.1002/(SICI)1522-2683(20000201)21:3<679::AID-ELPS679>3.0.CO;2-A

关键词: Electrospray ionizationSodium dodecyl sulfateCathepsin DGel electrophoresisMass spectrometryMolecular biologyProtein digestionTwo-dimensional gel electrophoresisIsoelectric focusingChemistry

摘要: … Tumor progression can be evaluated via examination of the expression of proteins for which the quantity varies according to tumor type and development (marker proteins). In this …

参考文章(21)
Akira Ichikawa, Hirofumi Tachibana, Seiji Kawamoto, Masanori Kamei, Tsutomu Honjoh, Shuichi Hashizume, Sanetaka Shirahata, Cytokeratin 8 and 19 as antigens recognized by adenocarcinoma-reactive human monoclonal antibody AE6F4 Human antibodies. ,vol. 8, pp. 195- 202 ,(1997) , 10.3233/HAB-1997-8405
J I Garrels, The QUEST system for quantitative analysis of two-dimensional gels. Journal of Biological Chemistry. ,vol. 264, pp. 5269- 5282 ,(1989) , 10.1016/S0021-9258(18)83728-0
R. Lotan, Xiao-Chun Xu, A. K. El-Naggar, Differential expression of galectin-1 and galectin-3 in thyroid tumors. Potential diagnostic implications. American Journal of Pathology. ,vol. 147, pp. 815- 822 ,(1995)
Giulio Francia, Stephen D. Mitchell, Stephen E. Moss, John F. Marshall, Ian R. Hart, Andrew M. Hanby, Identification by Differential Display of Annexin-VI, a Gene Differentially Expressed during Melanoma Progression Cancer Research. ,vol. 56, pp. 3855- 3858 ,(1996)
Peter Roepstorff, Mass spectrometry in protein studies from genome to function Current Opinion in Biotechnology. ,vol. 8, pp. 6- 13 ,(1997) , 10.1016/S0958-1669(97)80151-6
Guang Li, Mark Waltham, N. Leigh Anderson, Edward Unsworth, Anthony Treston, John N. Weinstein, Rapid mass spectrometric identification of proteins from two-dimensional polyacrylamide gels after in gel proteolytic digestion. Electrophoresis. ,vol. 18, pp. 391- 402 ,(1997) , 10.1002/ELPS.1150180313
Frédéric A Van Den Brûle, Crina Buicu, Andy Berchuck, Robert C Bast, Manuel Deprez, Fu-tong Liu, Douglas N.W Cooper, Claudette Pieters, Mark E Sobel, Vincent Castronovo, Expression of the 67-kD laminin receptor, galectin-1, and galectin-3 in advanced human uterine adenocarcinoma Human Pathology. ,vol. 27, pp. 1185- 1191 ,(1996) , 10.1016/S0046-8177(96)90313-5
Annette C. Kuesel, Thérèse Kroft, Michel Préfontaine, Ian C. P. Smith, Lipoprotein(a) and CA125 levels in the plasma of patients with benign and malignant ovarian disease International Journal of Cancer. ,vol. 52, pp. 341- 346 ,(1992) , 10.1002/IJC.2910520302
William W. Wong, Srinivasan Vijayakumar, Ralph R. Weichselbaum, Prognostic indicators in node-negative early stage breast cancer The American Journal of Medicine. ,vol. 92, pp. 539- 548 ,(1992) , 10.1016/0002-9343(92)90751-V