Cytokeratin 8 and 19 as antigens recognized by adenocarcinoma-reactive human monoclonal antibody AE6F4
作者： Akira Ichikawa , Hirofumi Tachibana , Seiji Kawamoto , Masanori Kamei , Tsutomu Honjoh
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摘要: The human monoclonal antibody (MAb) AE6F4 is secreted by a human-human hybridoma line established from the in vitro immunization of normal peripheral blood lymphocytes with lung adenocarcinoma cell line, A549. This MAb strongly reactive to cancer tissues. In previous study, antigens recognized were purified A549 cells and identified as /4-3-3 protein 31 kDa cytosolic phospholipase A2 (cP LA2)' also binds two kinds (53 40 kDa), which are not related or cPLA2, breast MCF-7. We 38 antigen, degradation product 53 antigen MCF-7 using ion-exchange hydroxyapatite column chromatography. Two partial amino acid sequences showed 95-100% homology cytokeratin 8 (CK8). Two-dimensional gel electrophoresis immunoblot analysis intermediate filament fraction separated demonstrated that CK8 CK19, respectively. Antigenic determinants on CK19 resistant sodium periodate treatment, although antigenic determinant (14-3-3 and( or) cP LA2) was, sensitive this treatment. These results suggest reacts both carbohydrate peptide determinants. [Hum Antibod 1997; 8: 195-202]
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sci-hub.se 参考文章(16)
Hiroharu Kawahara, Sanetaka Shirahata, Hirofumi Tachibana, Hiroki Murakami, In vitro immunization of human lymphocytes with human lung cancer cell line A549 Human antibodies and hybridomas. ,vol. 3, pp. 8- 13 ,(1992) , 10.3233/HAB-1992-3102
Paul. J. Donald, Robert D. Cardiff, Da-Er He, Katherine Kendall, Monoclonal antibody-porphyrin conjugate for head and neck cancer: the possible magic bullet. Otolaryngology-Head and Neck Surgery. ,vol. 105, pp. 781- 787 ,(1991) , 10.1177/019459989110500603
M W Cunningham, A R Shikhman, Immunological mimicry between N-acetyl-beta-D-glucosamine and cytokeratin peptides. Evidence for a microbially driven anti-keratin antibody response. Journal of Immunology. ,vol. 152, pp. 4375- 4387 ,(1994)
PH O'Farrell, High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry. ,vol. 250, pp. 4007- 4021 ,(1975) , 10.1016/S0021-9258(19)41496-8
Neil S. Greenspan, Madeleine W. Cunningham, Alexander R. Shikhman, Cytokeratin peptide SFGSGFGGGY mimics N-acetyl-beta-D-glucosamine in reaction with antibodies and lectins, and induces in vivo anti-carbohydrate antibody response. Journal of Immunology. ,vol. 153, pp. 5593- 5606 ,(1994)
Edmond Godfroid, Isabelle Parent, Claude Szpirer, Maurice Geuskens, Th. Dupressoir, Cytokeratins are exposed on the outer surface of established human mammary carcinoma cells. Journal of Cell Science. ,vol. 99, pp. 595- 607 ,(1991) , 10.1242/JCS.99.3.595
Paul V. Rossitto, Robert D. Cardiff, Benjamin F. Edwards, Raymond Chan, Presence of Proteolytically Processed Keratins in the Culture Medium of MCF-7 Cells Cancer Research. ,vol. 46, pp. 6353- 6359 ,(1986)
G. KÖHLER, C. MILSTEIN, Continuous cultures of fused cells secreting antibody of predefined specificity Nature. ,vol. 256, pp. 495- 497 ,(1975) , 10.1038/256495A0
M.P. Woodward, W.W. Young, R.A. Bloodgood, Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation Journal of Immunological Methods. ,vol. 78, pp. 143- 153 ,(1985) , 10.1016/0022-1759(85)90337-0
Seiji Kawamoto, Masahiro Shoji, Yuko Setoguchi, Masatoshi Kato, Shuichi Hashizume, Akira Ichikawa, Kazuhiro Osada, Yoshinori Katakura, Hirofumi Tachibana, Hiroki Murakami, Molecular cloning of the 31 kDa cytosolic phospholipase A2, as an antigen recognized by the lung cancer-specific human monoclonal antibody, AE6F4. Cytotechnology. ,vol. 17, pp. 103- 108 ,(1995) , 10.1007/BF00749397